Triphosphopyridine Nucleotide Diaphorase from Wheat Germ.

Triphosphopyridine Nucleotide Diaphorase from Wheat Germ.

11. KREBS, H. A. and HENSELEIT, K. Untersuchungen iiber die harnstoffbildung im tierkorper. Zeit. physiol. Chem. 210: 33. 1932. 12. McELROY, W. D. and GLASS, H. B. Amino acid Metabolism. Pp. 300-320. Johns Hopkins Press, Baltimore 1955. 13. MUELLER, G. C., QUINN, E. M. and RUECKERT, R. R. The formation of 1-phosphoerythrulose-4-C" by homogenates of Swiss chard leaves. Arch. Biochem. Biophys. 55...

The Histochemical Localization of Triphosphopyridine Nucleotide Diaphorase

A histochemical method is described for the localization of triphosphopyridine nucleotide diaphorase using a recently synthesized tetrazolium salt (Nitro-BT). By virtue of the favorable histochemical properties of this reagent, it has been possible to demonstrate that whereas DPN diaphorase is usually restricted to the mitochondria, the TPN diaphorase activity of corresponding cells was distrib...

Studies on an Enzyme System from Wheat Germ Catalyzing the Aerobic Oxidation of Reduced Triphosphopyridine Nucleotide.

The aerobic oxidation of reduced triphosphopyridine nucleotide by a wheat germ enzyme system.

Demonstration of TPNH Oxidase-The rate of decrease in light absorption at 340 rnp, due to TPNH, was used as an assay for the enzyme. The test was run routinely in glycylglycine buffer of pH 7.4. The conditions are given in detail in the legend to Fig. 1. Under these circumstances, the rate of change in optical density was roughly proportional to the amount of enzyme employed, in the range A log...

Preparation of triphosphopyridine nucleotide.